Large-Scale Synthesis of CISe/ZnS Core-Shell Quantum Dots and Its Effects on the Enzymatic Activity of Recombinant Human Furin (an Activator of SARS-CoV-2 S1/S2 Spike Proteins)

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dc.contributor.authorNcapayi, Vuyelwa
dc.contributor.authorFamutimi, Oladoyin Grace
dc.contributor.authorLebepe, Thabang Calvin
dc.contributor.authorMaluleke, Rodney
dc.contributor.authorMasha, Sam
dc.contributor.authorMgedle, Nande
dc.contributor.authorParani, Sundararajan
dc.contributor.authorKodama, Tetsuya
dc.contributor.authorAdewale, Isaac Olusanjo
dc.contributor.authorOluwafemi, Oluwatobi Samuel
dc.date.accessioned2024-05-07T11:47:28Z
dc.date.available2024-05-07T11:47:28Z
dc.date.issued2023
dc.description.abstractWe herein report, for the first time, the activity of copper indium selenide/zinc sulphide core-shell quantum dots (CISe/ZnS QDs) as an inhibitor against recombinant human furin, an enzyme that has been implicated in the aetiology of many diseases, including Covid-19. The cell viability of the as-synthesised CISe/ZnS QDs was tested against mouse colon carcinoma cells (C26), while the Furin activity was measured by hydrolysis of peptide substrate Pyr-RTKR-AMC liberating the fluorogenic 7-amino-4-methyl coumarin. The result showed that the as-synthesised stable near-infrared emitting (840 nm) CISe-ZnS QDs is biocompatible against C26 and can inhibit furin with an inhibition constant, Ki, of 15.66 μM. The IC50 was 11.29 ± 0.54 μM, while the enzymatic activity was abolished at about 23 μM of the inhibitor concentration. The results indicate the chemotherapeutic potential of CISe-ZnS QDs as an enzyme inhibitor, which may find application in managing diseases whose pathogenesis involves hyperactivity of furin.
dc.identifier.doihttps://doi.org/10.1016/j.colcom.2023.100737
dc.identifier.urihttps://africarxiv.ubuntunet.net/handle/1/1475
dc.identifier.urihttps://doi.org/10.60763/africarxiv/1415
dc.identifier.urihttps://doi.org/10.60763/africarxiv/1415
dc.identifier.urihttps://doi.org/10.60763/africarxiv/1415
dc.titleLarge-Scale Synthesis of CISe/ZnS Core-Shell Quantum Dots and Its Effects on the Enzymatic Activity of Recombinant Human Furin (an Activator of SARS-CoV-2 S1/S2 Spike Proteins)

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Research: COVID-19 Virus